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tpc2 mcherry plasmid  (Addgene inc)


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    Addgene inc tpc2 mcherry plasmid
    Tpc2 Mcherry Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tpc2+mcherry+plasmid/pm38263578-69-10-23?v=Addgene+inc
    Average 92 stars, based on 3 article reviews
    tpc2 mcherry plasmid - by Bioz Stars, 2026-06
    92/100 stars

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    Fig. 2. TPC activity is required for phagosome resolution. (A and B) RAW 264.7 cells expressing TPC1-tdTomato or TPC2-mCherry challenged with Alexa Fluor 488 anti-IgG labeled sRBC for indicated times. xy plane, Left. Extended focus, Left. (Scale bar, 5 µm.) Quantification in (B) for individual phagosomes. n = 3. (C and D) wt and TPC1/2−/− BMDM challenged with sRBC. Resolution assessed up to 4 h. (Scale bar, 10 µm.) Phagosome volume quantified in (D). Dots represent individual fields containing >3 cells, >9 phagosomes. n = 3. (E) As in (C), using 25× magnification. (Scale bar, 25 µm.) (F) wt and TPC1/2−/− BMDM and TPC1/2−/− BMDM expressing <t>wtTPC2-mCherry</t> or TPC2(N257A)-mCherry, quantified as in (D). Dots represent individual phagosomes. >30 cells, >100 phagosomes. n = 2.
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    Fig. 2. TPC activity is required for phagosome resolution. (A and B) RAW 264.7 cells expressing TPC1-tdTomato or TPC2-mCherry challenged with Alexa Fluor 488 anti-IgG labeled sRBC for indicated times. xy plane, Left. Extended focus, Left. (Scale bar, 5 µm.) Quantification in (B) for individual phagosomes. n = 3. (C and D) wt and TPC1/2−/− BMDM challenged with sRBC. Resolution assessed up to 4 h. (Scale bar, 10 µm.) Phagosome volume quantified in (D). Dots represent individual fields containing >3 cells, >9 phagosomes. n = 3. (E) As in (C), using 25× magnification. (Scale bar, 25 µm.) (F) wt and TPC1/2−/− BMDM and TPC1/2−/− BMDM expressing <t>wtTPC2-mCherry</t> or TPC2(N257A)-mCherry, quantified as in (D). Dots represent individual phagosomes. >30 cells, >100 phagosomes. n = 2.
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    Fig. 2. TPC activity is required for phagosome resolution. (A and B) RAW 264.7 cells expressing TPC1-tdTomato or TPC2-mCherry challenged with Alexa Fluor 488 anti-IgG labeled sRBC for indicated times. xy plane, Left. Extended focus, Left. (Scale bar, 5 µm.) Quantification in (B) for individual phagosomes. n = 3. (C and D) wt and TPC1/2−/− BMDM challenged with sRBC. Resolution assessed up to 4 h. (Scale bar, 10 µm.) Phagosome volume quantified in (D). Dots represent individual fields containing >3 cells, >9 phagosomes. n = 3. (E) As in (C), using 25× magnification. (Scale bar, 25 µm.) (F) wt and TPC1/2−/− BMDM and TPC1/2−/− BMDM expressing wtTPC2-mCherry or TPC2(N257A)-mCherry, quantified as in (D). Dots represent individual phagosomes. >30 cells, >100 phagosomes. n = 2.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Two-pore channels regulate endomembrane tension to enable remodeling and resolution of phagolysosomes.

    doi: 10.1073/pnas.2309465121

    Figure Lengend Snippet: Fig. 2. TPC activity is required for phagosome resolution. (A and B) RAW 264.7 cells expressing TPC1-tdTomato or TPC2-mCherry challenged with Alexa Fluor 488 anti-IgG labeled sRBC for indicated times. xy plane, Left. Extended focus, Left. (Scale bar, 5 µm.) Quantification in (B) for individual phagosomes. n = 3. (C and D) wt and TPC1/2−/− BMDM challenged with sRBC. Resolution assessed up to 4 h. (Scale bar, 10 µm.) Phagosome volume quantified in (D). Dots represent individual fields containing >3 cells, >9 phagosomes. n = 3. (E) As in (C), using 25× magnification. (Scale bar, 25 µm.) (F) wt and TPC1/2−/− BMDM and TPC1/2−/− BMDM expressing wtTPC2-mCherry or TPC2(N257A)-mCherry, quantified as in (D). Dots represent individual phagosomes. >30 cells, >100 phagosomes. n = 2.

    Article Snippet: Mammalian expression vectors were described in the following sources: wtTPC1- tdTomato (13), wtTPC2- mCherry (Addgene; plasmid no. 135183), TPC2(N257A)- mCherry (Addgene; plasmid no. 135186), LAMP1- GFP (35), and EEA1- eGFP (77).

    Techniques: Activity Assay, Expressing, Labeling